A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1

Staphylococcus women ISK-1 produces a (antibiotic, nukacin ISK-1. The nukacin ISK-1 gene cluster consists of at least six genes, nukA, -M, -T, -F, -E, and -G, and two open reading frames, ORF1 and ORF7 (designated nukH). Sequence comparisons suggested that NukF, -E, -G, and -H contribute to immunity...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005, Vol.69 (7), p.1403-1410
Main Authors: Aso, Y. (Kyushu Univ., Fukuoka (Japan)), Okuda, K, Nagao, J, Kanemasa, Y, Phuong, N.T.B, Koga, H, Shioya, K, Sashihara, T, Nakayama, J, Sonomoto, K
Format: Article
Language:English
Subjects:
ANTIBIOTICS
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
bacteriocin
BACTERIOCINS
Bacteriocins - immunology
Bacteriocins - metabolism
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
GENES
IMMUNITY
LACTOCOCCUS LACTIS
Lactococcus lactis - genetics
Lactococcus lactis - immunology
Lactococcus lactis - metabolism
lantibiotic
Membrane Proteins - genetics
Membrane Proteins - metabolism
Multigene Family - genetics
Nisin - immunology
Nisin - metabolism
NUCLEOTIDE SEQUENCE
nukacin ISK-1
PEPTIDES
Protein Binding
PROTEINS
Recombinant Proteins - metabolism
RECOMBINATION
STAPHYLOCOCCUS
Staphylococcus - genetics
Staphylococcus - immunology
Staphylococcus - metabolism
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Summary:Staphylococcus women ISK-1 produces a (antibiotic, nukacin ISK-1. The nukacin ISK-1 gene cluster consists of at least six genes, nukA, -M, -T, -F, -E, and -G, and two open reading frames, ORF1 and ORF7 (designated nukH). Sequence comparisons suggested that NukF, -E, -G, and -H contribute to immunity to nukacin ISK-1. We investigated the immunity levels of recombinant Lactococcus lactis expressing nukFEG and nukH against nukacin ISK-1. The co-expression of nukFEG and nukH resulted in a nigh degree of immunity. The expression of either nuk-FEG or nukH conferred partial immunity against nukacin ISK-1. These results suggest that NukH contributes cooperatively to self-protection with NukFEG. The nukacin ISK-1 immunity system might function against another (antibiotic, lacticin 481. Western blot analysis showed that NukH expressed in Staphylococcus carnosus was localized in the membrane. Peptide release/bind assays indicated that the recombinant L, lactis expressing nukH interacted with nukacin ISK-1 and lacticin 481 but not with nisin A. These findings suggest that NukH contributes cooperatively to host immunity as a novel type of lantibiotic-binding immunity protein with NukFEG.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.1403