Effect of various antagonists on the Channa striatus fillet extract antinociception in mice

• Published in: Canadian journal of physiology and pharmacology 2005-07, Vol.83 (7), p.635-642
• Main Authors: Zakaria, Z A, Sulaiman, M R, Jais, A M. Mat, Somchit, M N
• Format: Article
• Language: English
• Subjects: Amino acids; Analgesics; Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Aspirin - pharmacology; Biological and medical sciences; Diet; Dose-Response Relationship, Drug; Fatty acids; Fish; Fundamental and applied biological sciences. Psychology; Male; Medical applications; Mice; Mice, Inbred BALB C; Muscle Contraction - drug effects; Muscle, Skeletal - physiology; Naloxone - pharmacology; Narcotic Antagonists - pharmacology; Pain Measurement - drug effects; Perciformes - physiology; Reaction Time - drug effects; Receptors, Cell Surface - antagonists & inhibitors; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors; Tissue Extracts - antagonists & inhibitors; Tissue Extracts - physiology; Vertebrates: nervous system and sense organs; Wound healing
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/y05-050

The effects of an aqueous supernatant of haruan (ASH) (Channa striatus) fillet extract on various antinociception receptor system activities were examined using a mouse abdominal-constriction model. Mice that were pretreated with distilled water, s.c., followed 10 min later by administration of 25%, 50%, and 100% concentration ASH, s.c., produced a significant concentration-dependent antinociceptive activity (p < 0.001). Pretreatment with naloxone (0.3, 1.0, and 3.0 mg/kg body mass), 10 min before ASH administration, failed to block the extract antinociception. Pretreatment of the 100% concentration ASH with mecamylamine (5 mg/kg), pindolol (10 mg/kg), and haloperidol (1 mg/kg) also did not cause any significant change in its antinociception. However, pretreatment with atropine (5 mg/kg), bicuculline (10 mg/kg), phenoxybenzamine (10mg/kg), and methysergide (5 mg/kg) were found to reverse ASH antinociception. Based on the above findings, the ASH is suggested to contain different types of bioactive compounds that act synergistically on muscarinic, GABA A , α-adrenergic, and serotonergic receptor systems to produce the observed antinocicep tion. Key words: Haruan (Channa striatus), antinociceptive, writhing test, receptor systems