Epitope of the Vaccine-Type Bordetella pertussis Strain 186 Lipooligosaccharide and Antiendotoxin Activity of Antibodies Directed against the Terminal Pentasaccharide-Tetanus Toxoid Conjugate

• Published in: Infection and Immunity 2005-11, Vol.73 (11), p.7381-7389
• Main Authors: Niedziela, Tomasz, Letowska, Iwona, Lukasiewicz, Jolanta, Kaszowska, Marta, Czarnecka, Anna, Kenne, Lennart, Lugowski, Czeslaw
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - immunology; Bacteriology; Biological and medical sciences; Bordetella pertussis; Bordetella pertussis - classification; Bordetella pertussis - immunology; Carbohydrate Conformation; Cell Line; Endotoxins - immunology; Epitope Mapping; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Immune Sera - immunology; Immunoconjugates - chemistry; Immunoconjugates - immunology; Immunodominant Epitopes - chemistry; Immunodominant Epitopes - immunology; Immunodominant Epitopes - isolation & purification; Interleukin-6 - metabolism; Lipopolysaccharides - chemistry; Lipopolysaccharides - immunology; Lipopolysaccharides - isolation & purification; Macrophages - immunology; Microbial Immunity and Vaccines; Microbiology; Miscellaneous; Nitric Oxide - metabolism; Pertussis Vaccine - chemistry; Pertussis Vaccine - immunology; Rabbits; Tetanus Toxoid - chemistry; Tetanus Toxoid - immunology; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/iai.73.11.7381-7389.2005

Lipooligosaccharides (LOS) isolated from Bordetella pertussis strains 186 and 606 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-resolution magic angle spinning nuclear magnetic resonsnace (NMR). These analyses distinguished between the LOS of strains 186 and 606, suggesting that the structure of LOS in B. pertussis is heterogeneous. The pentasaccharide was selectively cleaved from LOS of B. pertussis strain 186, purified, and covalently linked to a monomer fraction of tetanus toxoid. Injection of rabbits with the neoglycoconjugate emulsified in complete Freund's adjuvant yielded immunoglobulin G antibodies that were reactive with the LOS. These antibodies reacted strongly with B. pertussis LOS possessing the complete dodecasaccharide, as determined by an enzyme-linked immunosorbent assay, immunoblotting, and flow cytometry with intact, live bacterial cells. The binding epitope within the pentasaccharide was investigated by saturation transfer difference (STD) NMR spectroscopy. Protons H-1 and H-4 of the terminal [alpha]-D-GlcpNAc and proton H-6 and protons of an N-methyl group at H-4 of 3-substituted {szligbeta}-L-FucpNAc4NMe exhibited the largest saturation transfers. STD NMR experiments confirmed that the immunodominant epitope recognized by the antineoglycoconjugate antibodies is located predominantly in the distal trisaccharide of B. pertussis 186 LOS. The antipentasaccharide antibodies induced by the conjugate inhibited the secretion of tumor necrosis factor alpha, interleukin-6, and NO by LOS-stimulated J774A.1 cells.