NEOSTRIATAL CYTOSKELETON CHANGES FOLLOWING PERINATAL ASPHYXIA: EFFECT OF HYPOTHERMIA TREATMENT

Long-term changes of different types of neurofilaments (NF) and glial fibrilar acid protein (GFAP) were studied in neostriatal rat subjected to perinatal asphyxia (PA) under normothermic and hypothermic (15°C) conditions, using immunohistochemistry for light and electron microscopy. Neostriatal neur...

Full description

Saved in:
Bibliographic Details
Published in:International journal of neuroscience 2006-06, Vol.116 (6), p.697-714
Main Authors: CEBRAL, ELISA, CAPANI, FRANCISCO, SELVÍN-TESTA, ASIA, FUNES, MANUEL REY, COIRINI, HÉCTOR, LOIDL, C. FABIÁN
Format: Article
Language:English
Subjects:
Analysis of Variance
Anatomical correlates of behavior
Animals
Animals, Newborn
Asphyxia - metabolism
Asphyxia - therapy
Behavioral psychophysiology
Biological and medical sciences
Cytoskeleton - metabolism
Disease Models, Animal
Female
Fundamental and applied biological sciences. Psychology
Glial Fibrillary Acidic Protein - metabolism
Hypothermia
Image Processing, Computer-Assisted - methods
Immunohistochemistry - methods
Male
Microscopy, Immunoelectron - methods
neostriatum
Neostriatum - metabolism
Neostriatum - ultrastructure
neurofilament
perinatal asphyxia
Pregnancy
Psychology. Psychoanalysis. Psychiatry
Psychology. Psychophysiology
Rats
Rats, Sprague-Dawley
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Long-term changes of different types of neurofilaments (NF) and glial fibrilar acid protein (GFAP) were studied in neostriatal rat subjected to perinatal asphyxia (PA) under normothermic and hypothermic (15°C) conditions, using immunohistochemistry for light and electron microscopy. Neostriatal neurons of 6-month-old rats that were subjected to 19 and 20 min of PA, showed an increase of NF 200 kDa immunostaining mainly in the axon fascicles in comparison with the control and hypothermia groups. In contrast, no alterations were seen with NF68 and NF160 neurofilament antibodies. Furthermore, the same PA groups showed astroglial cells with enhanced GFAP immunoreactivity, evidencing a typical astroglial reaction with a clear hypertrophy of these cells. A quantitative image analysis confirmed these observations. Hypothermic treated animals did show neither astroglial nor neuronal cytoskeletal changes in comparison to the control group. These findings showed that PA produces chronic cytoskeletal alterations in the neostriatum cells that can be prevented by hypothermia.
ISSN:0020-7454
1563-5279
1543-5245
DOI:10.1080/00207450600674970