Malate Biosensors for the Monitoring of Malolactic Fermentation: Different Approaches

Different strategies for L-malic acid monitoring in wine are presented in this paper. A bi-enzymatic sensor was described based on the coupling of L-malate dehydrogenase with NADHoxidase, while monoenzymatic systems were based either on L-malate dehydrogenase or malate-quinone oxidoreductase. The bi...

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Bibliographic Details
Published in:Analytical letters 2006-07, Vol.39 (8), p.1543-1558
Main Authors: Gurban, A.-M., Prieto-Simón, B., Marty, J.-L., Noguer, T.
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
biosensor
Biosensors
Biotechnology
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Malate dehydrogenase
malate-quinone oxidoreductase
Methods. Procedures. Technologies
NADHoxidase
Various methods and equipments
Vitaceae
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Summary:Different strategies for L-malic acid monitoring in wine are presented in this paper. A bi-enzymatic sensor was described based on the coupling of L-malate dehydrogenase with NADHoxidase, while monoenzymatic systems were based either on L-malate dehydrogenase or malate-quinone oxidoreductase. The bienzymatic sensors showed sensitivities of 3.6 mA/M and a detection limit of 4.5 µM, while for the monoenzymatic sensors the sensitivity was 1.1 mA/M and the detection limit was 2.4 µM. The optimized biosensors were used for determination of L-malic acid in different samples of wines.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710600713214