Porphyromonas gingivalis galE Is Involved in Lipopolysaccharide O-Antigen Synthesis and Biofilm Formation

• Published in: Infection and Immunity 2006-11, Vol.74 (11), p.6145-6153
• Main Authors: Nakao, Ryoma, Senpuku, Hidenobu, Watanabe, Haruo
• Format: Article
• Language: English
• Subjects: Biofilms; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Microbiology; Microscopy, Electron, Scanning; Molecular Pathogenesis; Mutation; O Antigens - biosynthesis; Penicillin Resistance - genetics; Porphyromonas gingivalis; Porphyromonas gingivalis - drug effects; Porphyromonas gingivalis - enzymology; Porphyromonas gingivalis - genetics; Porphyromonas gingivalis - ultrastructure; UDPglucose 4-Epimerase - deficiency; UDPglucose 4-Epimerase - genetics; UDPglucose 4-Epimerase - physiology
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00261-06

Porphyromonas gingivalis is a crucial component of complex plaque biofilms that form in the oral cavity, resulting in the progression of periodontal disease. To elucidate the mechanism of periodontal biofilm formation, we analyzed the involvement of several genes related to the synthesis of polysaccharides in P. gingivalis. Gene knockout P. gingivalis mutants were constructed by insertion of an ermF-ermAM cassette; among these mutants, the galE mutant showed some characteristic phenotypes involved in the loss of GalE activity. As expected, the galE mutant accumulated intracellular carbohydrates in the presence of 0.1% galactose and did not grow in the presence of galactose at a concentration greater than 1%, in contrast to the parental strain. Lipopolysaccharide (LPS) analysis indicated that the length of the O-antigen chain of the galE mutant was shorter than that of the wild type. It was also demonstrated that biofilms generated by the galE mutant had an intensity 4.5-fold greater than those of the wild type. Further, the galE mutant was found to be significantly susceptible to some antibiotics in comparison with the wild type. In addition, complementation of the galE mutation led to a partial recovery of the parental phenotypes. We concluded that the galE gene plays a pivotal role in the modification of LPS O antigen and biofilm formation in P. gingivalis and considered that our findings of a relationship between the function of the P. gingivalis galE gene and virulence phenotypes such as biofilm formation may provide clues for understanding the mechanism of pathogenicity in periodontal disease.