Effect of dietary cyclic nigerosylnigerose on intestinal immune functions in mice

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-10, Vol.70 (10), p.2481-2487
• Main Authors: Hino, K.(Hayashibara Biochemical Labs. Inc., Okayama (Japan)), Kurose, M, Sakurai, T, Inoue, S, Oku, K, Chaen, H, Kohno, K, Fukuda, S
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; cyclic nigerosylnigerose; Dietary Supplements; Feces; Fundamental and applied biological sciences. Psychology; Glucans - pharmacology; IMMUNE RESPONSE; Immunity - drug effects; immunoglobulin A (IgA); Immunoglobulin A - analysis; interleukin-6 (IL-6); Interleukin-6 - analysis; INTERLEUKINE; INTERLEUKINS; INTERLEUQUINAS; INTESTIN; Intestinal Mucosa - immunology; INTESTINES; Intestines - immunology; INTESTINOS; MICE; Mice, Inbred BALB C; OLIGOSACARIDOS; OLIGOSACCHARIDE; OLIGOSACCHARIDES; Oligosaccharides - pharmacology; Peyer's patch (PP); Peyer's Patches - immunology; Peyer's Patches - secretion; RATON; REPONSE IMMUNITAIRE; RESPUESTA INMUNOLOGICA; SOURIS; Transforming Growth Factor beta1 - analysis; transforming growth factor-β1 (TGF-β1)
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.60215

We examined the dietary effects of cyclic nigerosylnigerose (CNN), a dietary indigestible oligosaccharide with four D-glucopyranosyl residues linked by alternating alpha-(1-3)- and alpha-(1-6) glucosidic linkages, on the intestinal immune function of mice, and the effects were compared with those of alpha-(1-3)-linked oligosaccharide (nigerooligosaccharides, NOS) or alpha(1-6)-linked oligosaccharide (isomaltooligosaccharides, IMO). BALB/c mice were fed with 1-5% CNN, 5% IMO, or 12.5% NOS for 4 weeks, and the intestinal mucosal immune responses were determined. In the 1-5% CNN fed groups, the amounts of IgA in feces increased significantly. In addition, IgA, transforming growth factor-beta1 (TGF-beta1), and interleukin-6 (IL-6) secretion by Peyer's patch (PP) cells were enhanced in CNN fed mice. In the 5% CNN group, pH in the cecum decreased, and the amounts of lactic acid and butyric acid increased. These findings were not observed in the NOS- or IMO-fed group of mice. They suggest that CNN supplementation changes the intestinal environment of microflora and indirectly enhances the immune function in the gut.