Rhythmic cycle of clathrin-coated pit formation at the trans-Golgi network in human MDA-MB-435 cells

We studied the in vivo dynamics of enhanced green fluorescent protein-tagged clathrin light chain a (GFP-CLCa) at the trans-Golgi network (TGN) in MDA-MB-435 cells. The intensity of fluorescence signals of GFPCLCa periodically increased and decreased at the TGN approximately every 100 s. This sugges...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2007-02, Vol.71 (2), p.571-574
Main Authors: Sakaushi, S.(Osaka Prefectural Univ., Sakai (Japan)), Senda Murata, K, Fukada, T, Oka, S, Sugimoto, K
Format: Article
Language:English
Subjects:
Animals
BIOCHEMISTRY
BIOCHIMIE
Biological and medical sciences
BIOQUIMICA
brefeldin A (BFA)
Cell Line
Cell Membrane - metabolism
Cell Membrane - ultrastructure
CELL WALLS
CELLS
CELLULE
CELULAS
Clathrin - biosynthesis
clathrin light chain a (CLCa)
FLUORESCENCE
FLUORESCENCIA
Fundamental and applied biological sciences. Psychology
GENERO HUMANO
GENRE HUMAIN
Golgi apparatus
Green Fluorescent Proteins - biosynthesis
Green Fluorescent Proteins - genetics
Humans
live-imaging microscopy
MANKIND
Mice
MICROSCOPIA
MICROSCOPIE
MICROSCOPY
Microscopy, Fluorescence
Microscopy, Video
Molecular Sequence Data
NIH 3T3 Cells
PARED CELULAR
PAROI CELLULAIRE
periodic fluctuations (PFs)
PERIODICIDAD
PERIODICITE
PERIODICITY
PROTEINAS
PROTEINE
PROTEINS
trans-Golgi Network - metabolism
trans-Golgi Network - ultrastructure
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied the in vivo dynamics of enhanced green fluorescent protein-tagged clathrin light chain a (GFP-CLCa) at the trans-Golgi network (TGN) in MDA-MB-435 cells. The intensity of fluorescence signals of GFPCLCa periodically increased and decreased at the TGN approximately every 100 s. This suggests that the formation of clathrin-coated pits occurs synchronously and periodically at the TGN.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60434