High-performance liquid chromatographic separation of carbohydrates on stationary phases prepared from polystyrene-based resin and tertiary amines: Effect of chemical structure of anion-exchange sorbents

Two types of new anion-exchange stationary phases were readily prepared by the reaction of porous particles of chloromethylated styrene–divinylbenzene copolymer with various amines. The stationary phases M n , where n is the length of alkyl chain, were obtained by the reaction with N, N-dimethylalky...

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Bibliographic Details
Published in:Journal of Chromatography A 1999-06, Vol.845 (1), p.401-408
Main Authors: Masuda, Takako, Nishimura, Yukihiro, Tonegawa, Masami, Kitahara, Keiichi, Arai, Sadao, Yamashita, Junzo, Takai, Nobuharu
Format: Article
Language:English
Subjects:
Analytical chemistry
Carbohydrates
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:Two types of new anion-exchange stationary phases were readily prepared by the reaction of porous particles of chloromethylated styrene–divinylbenzene copolymer with various amines. The stationary phases M n , where n is the length of alkyl chain, were obtained by the reaction with N, N-dimethylalkylamines. The reaction with N, N, N′, N′-tetramethyldiaminoalkanes also afforded the stationary phases D n with a terminal functional group, where n is the length of methylene bridge. The HPLC separations of monosaccharides, disaccharides and oligosaccharides were successfully performed on these stationary phases using electrochemical detection with a Ni–Ti alloy working electrode in alkaline eluents. The effects of chemical structures of stationary phases on the separation of carbohydrates are described.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00243-5