Proteomic Characterization of Tissue Expansion of Rice Scutellum Stimulated by Abscisic Acid

We found that appropriate treatment with a highly potent and long-lasting abscisic acid analog enhanced the tissue expansion of scutellum during early seedling development of rice, accompanied by increases of protein and starch accumulation in the tissue. A comparative display of the protein express...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2007-05, Vol.71 (5), p.1260-1268
Main Authors: ASAKURA, Tsuyoshi, HIROSE, Shota, ASATSUMA, Satoru, NANJO, Yohei, NAKAIZUMI, Tetsuyo, ITOH, Kimiko, HORI, Hidetaka, KOMATSU, Setsuko, MITSUI, Toshiaki
Format: Article
Language:English
Subjects:
abscisic acid (ABA)
Abscisic Acid - analogs & derivatives
Abscisic Acid - analysis
Abscisic Acid - pharmacology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
germination
Oryza - drug effects
Oryza - growth & development
Oryza - metabolism
Oryza sativa
Plant Growth Regulators - analysis
Plant Growth Regulators - pharmacology
Plant Proteins - analysis
Plant Proteins - metabolism
proteome
Proteome - analysis
Proteomics - methods
Reference Standards
scutellum
Seedlings - drug effects
Seedlings - metabolism
Starch - biosynthesis
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Summary:We found that appropriate treatment with a highly potent and long-lasting abscisic acid analog enhanced the tissue expansion of scutellum during early seedling development of rice, accompanied by increases of protein and starch accumulation in the tissue. A comparative display of the protein expression patterns in the abscisic acid analog-treated and non-treated tissues on two dimensional gel electrophoretogram indicated that approximately 30% of the scutellar proteins were induced by abscisic acid. The abscisic acid-induced proteins included sucrose metabolizing, glycolytic, and ATP-producing enzymes. Most of these enzyme proteins also increased during the seedling growth. In addition, the expression of some isoforms of UDP-glucose pyrophosphorylase, 3-phosphoglycerate kinase, and mitochondrial ATP synthase beta chain was stimulated in the scutellum, with suppressed expression of α-amylase. We concluded that abscisic acid directly and indirectly stimulates the expression of numerous proteins, including carbohydrate metabolic enzymes, in scutellar tissues.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60675