Fluorimetric method for the determination of thiocyanate with 2′,7′-dichlorofluorescein and iodine

A highly sensitive and selective fluorimetric method has been developed for the determination of thiocyanate with 2′,7′-dichlorofluorescein ( λ ex = 508 nm, λ em = 524 nm) at pH 5.8–6.7. The calibration graph is linear over the range 1.0–60 ng ml −1 SCN −. The detection limit is 1.0 ng ml −1 SCN −....

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Bibliographic Details
Published in:Analytica chimica acta 1999-08, Vol.394 (2), p.171-175
Main Authors: Gong, Bolin, Gong, Guoquan
Format: Article
Language:English
Subjects:
2′,7′-dichlorofluorescein
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fluorimetry
Fundamental and applied biological sciences. Psychology
Intermediary metabolites. Miscellaneous
Iodine
Other biological molecules
Saliva
Serum
Thiocyanate
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Summary:A highly sensitive and selective fluorimetric method has been developed for the determination of thiocyanate with 2′,7′-dichlorofluorescein ( λ ex = 508 nm, λ em = 524 nm) at pH 5.8–6.7. The calibration graph is linear over the range 1.0–60 ng ml −1 SCN −. The detection limit is 1.0 ng ml −1 SCN −. The method has been successfully applied to the determination of thiocyanate ion in saliva and serum.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(99)00295-0