Development and Validation of High-Performance Liquid Chromatographic Method for Estimation of Lercanidipine in Rabbit Serum

A new, simple, and sensitive reverse-phase liquid chromatographic method was developed and validated for the estimation of Lercanidipine hydrochloride in rabbit serum using UV detector under isocratic conditions. After subjecting serum to simple and efficient one-step extraction procedure, 100 µl of...

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Bibliographic Details
Published in:Analytical letters 2007-01, Vol.40 (11), p.2128-2140
Main Authors: Charde, Shrikant, Kumar, Lajwinder, Saha, Ranendra
Format: Article
Language:English
Subjects:
1,4-dihydropyridine
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Detectors
Exact sciences and technology
Guidelines
high-performance liquid chromatography
Lercanidipine hydrochloride
Liquids
Mathematical analysis
Other chromatographic methods
rabbit serum
Rabbits
Serums
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Summary:A new, simple, and sensitive reverse-phase liquid chromatographic method was developed and validated for the estimation of Lercanidipine hydrochloride in rabbit serum using UV detector under isocratic conditions. After subjecting serum to simple and efficient one-step extraction procedure, 100 µl of sample was injected onto high-performance liquid chromatography system. The detector response was linear in the concentration range of 25-1000 ng/ml. The developed method was validated as per standard guidelines. Validation demonstrated accuracy, precision, and selectivity of the proposed method. The drug was found to be stable under various processing and storage conditions.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710701566636