Tissue Specific Expression of Avian Vitellogenin Gene is Correlated with DNA Hypomethylation and in vivo Specific Protein-DNA Interactions

The avian vitellogenin gene is expressed only in the liver of egg-laying hens. It can, however, be activated in immature chicks or roosters by oestradiol. Parallel to the onset of transcription, there is a demethylation of specific mCpGs in the promoter region and in the oestrogen response element (...

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Bibliographic Details
Published in:Philosophical transactions of the Royal Society of London. Series B, Biological sciences Biological sciences, 1990-01, Vol.326 (1235), p.231-240
Main Authors: Jost, J. P., Saluz, H. P., McEwan, I., Feavers, I. M., Hughes, M., Reiber, S., Liang, H. M., Vaccaro, M.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Chickens
DNA
DNA - genetics
DNA-Binding Proteins - metabolism
Estradiol - pharmacology
Estrogens
Female
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes - drug effects
Genomics
Hypersensitivity
Liver
Liver - metabolism
Male
Messenger RNA
Methylation
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Oviposition
Promoter regions
Promoter Regions, Genetic
Receptors
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
Sequencing
Transcription, Genetic - drug effects
Vitellogenins - genetics
Xenopus laevis
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Summary:The avian vitellogenin gene is expressed only in the liver of egg-laying hens. It can, however, be activated in immature chicks or roosters by oestradiol. Parallel to the onset of transcription, there is a demethylation of specific mCpGs in the promoter region and in the oestrogen response element (ERE). The methylation pattern in the promoter region is hormone and expression specific, whereas in the ERE it is only hormone and not organ specific. The demethylation occurring in the promoter region is correlated with the appearance of DNase I hypersensitivity sites and changes in the specific protein-DNA interactions. In vivo genomic footprinting of the ERE with varying concentrations of dimethylsulphate revealed, upon gene activation, only minor changes in the protein-DNA interaction. We present evidence that there is another protein that binds with high affinity to the ERE, besides the oestrogen receptor.
ISSN:0962-8436
0080-4622
1471-2970
2054-0280
DOI:10.1098/rstb.1990.0007