Corn metabolites affect growth and virulence of Agrobacterium tumefaciens

Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-labile inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1990-05, Vol.87 (10), p.3879-3883
Main Authors: Sahi, S.V. (North Carolina State University, Raleigh, NC), Chilton, M.D, Chilton, W.S
Format: Article
Language:English
Subjects:
AGROBACTERIUM
Aqueous solutions
Bacteriology
Biological and medical sciences
Corn
CRECIMIENTO
CROISSANCE
Fundamental and applied biological sciences. Psychology
Gene induction
GROWTH
METABOLITE
METABOLITES
METABOLITOS
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Plant gall
Plant growth
Plant tumor inducing plasmids
PLANTULAS
PLANTULE
SEEDLINGS
TRANSFORMATIONS
VIRULENCE
VIRULENCIA
ZEA MAYS
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Summary:Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-labile inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings (B73) at a concentration of 1.5 mM or greater. A concentration of 0.3 mM DIMBOA is sufficient to block growth of A. tumefaciens completely for 220 hr. DIMBOA at 0.1 mM concentration completely inhibited vir gene induction by 100 μ M AS and reduced growth rate by 50%. Thus, DIMBOA can be expected to have a significant effect on attempts to transform corn by using A. tumefaciens as a vector.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.87.10.3879