Nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein

The nit-2 major nitrogen regulatory gene of Neurospora crassa turns on the expression of various unlinked structural genes that specify nitrogen-catabolic enzymes under nitrogen-limitation conditions. The nit-2 gene encodes a protein of 1036 amino acid residues with a single zinc finger and a downst...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1990-07, Vol.87 (14), p.5331-5335
Main Authors: Fu, Y.H. (Ohio State University, Columbus, OH), Marzluf, G.A
Format: Article
Language:English
Subjects:
ADN
Amino acids
Aspergillus
Base Sequence
Binding Sites
Biological and medical sciences
CHAMPIGNON
Cloning, Molecular
CODE GENETIQUE
CODIGO GENETICO
DNA
DNA-binding protein
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
DNA-Binding Proteins - isolation & purification
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
FUNGI
GALACTOSIDASA
GALACTOSIDASE
GALACTOSIDASES
GENE
Gene Expression
GENES
Genes, Bacterial
Genes, Fungal
Genes. Genome
GENETIC CODE
Genetic vectors
Metalloproteins - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Neurospora
Neurospora - genetics
Neurospora crassa
Neurospora crassa - genetics
Neurospora crassa - metabolism
Nitrogen
Nitrogen Fixation - genetics
Promoter regions
Promoter Regions, Genetic
PROTEINAS
PROTEINE
PROTEINS
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - isolation & purification
Restriction Mapping
Sequence Homology, Nucleic Acid
Citations: Items that cite this one
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Description
Summary:The nit-2 major nitrogen regulatory gene of Neurospora crassa turns on the expression of various unlinked structural genes that specify nitrogen-catabolic enzymes under nitrogen-limitation conditions. The nit-2 gene encodes a protein of 1036 amino acid residues with a single zinc finger and a downstream basic region that may make up a DNA-binding domain. The zinc-finger domain of the NIT2 protein was synthesized in two ways to examine its DNA-binding activity with gel-band-mobility shift and DNA-footprint experiments. The NIT2 protein binds to specific DNA recognition elements that are located upstream of nitrogen-regulated structural genes. Each recognition element contains at least two copies of a core sequence whose consensus is TATCTA.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.87.14.5331