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Protein Kinase C is Present in Human Sperm: Possible Role in Flagellar Motility
We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased f...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1990-09, Vol.87 (18), p.7305-7308 |
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| container_end_page | 7308 |
| container_issue | 18 |
| container_start_page | 7305 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 87 |
| creator | Rotem, Ronit Paz, Gedalia F. Homonnai, Zvi T. Kalina, Moshe Naor, Zvi |
| description | We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm. |
| doi_str_mv | 10.1073/pnas.87.18.7305 |
| format | article |
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PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.87.18.7305</identifier><identifier>PMID: 2402510</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine ; 560300 - Chemicals Metabolism & Toxicology ; Adult ; Alkaloids - pharmacology ; Analytical, structural and metabolic biochemistry ; ANTIBODIES ; Antibodies, Monoclonal ; ATP ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; BIOCHEMISTRY ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; CARCINOGENS ; Cell biology ; CHEMISTRY ; CYTOCHEMISTRY ; DAYS LIVING RADIOISOTOPES ; Delta cells ; ENZYME ACTIVITY ; ENZYMES ; Enzymes and enzyme inhibitors ; ESTERS ; flagella ; Fundamental and applied biological sciences. Psychology ; GAMETES ; GERM CELLS ; Homogenization ; Humans ; Immunohistochemistry ; Isoquinolines - pharmacology ; ISOTOPES ; Kinetics ; LIGHT NUCLEI ; Male ; man ; MONOCLONAL ANTIBODIES ; motility ; NUCLEI ; NUCLEOTIDES ; ODD-ODD NUCLEI ; ORGANIC COMPOUNDS ; PHORBOL ESTERS ; PHOSPHORUS 32 ; PHOSPHORUS ISOTOPES ; PHOSPHORUS-GROUP TRANSFERASES ; PHOSPHOTRANSFERASES ; Piperazines - pharmacology ; Polyclonal antibodies ; protein kinase C ; Protein Kinase C - analysis ; Protein Kinase C - antagonists & inhibitors ; Protein Kinase C - metabolism ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIOISOTOPES ; Rats ; Reference Values ; requirements ; Sperm motility ; Sperm Motility - drug effects ; SPERMATOZOA ; Spermatozoa - drug effects ; Spermatozoa - enzymology ; Spermatozoa - physiology ; Sphingosine - pharmacology ; Staurosporine ; Tetradecanoylphorbol Acetate - pharmacology ; TRANSFERASES</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1990-09, Vol.87 (18), p.7305-7308</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-574a2be87908326993935a63583ad4e384e49dc4805a6a0d1c20d3faabe367bc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/87/18.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2355213$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2355213$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792,58237,58470</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19545610$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2402510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5447998$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Rotem, Ronit</creatorcontrib><creatorcontrib>Paz, Gedalia F.</creatorcontrib><creatorcontrib>Homonnai, Zvi T.</creatorcontrib><creatorcontrib>Kalina, Moshe</creatorcontrib><creatorcontrib>Naor, Zvi</creatorcontrib><title>Protein Kinase C is Present in Human Sperm: Possible Role in Flagellar Motility</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.</description><subject>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine</subject><subject>560300 - Chemicals Metabolism & Toxicology</subject><subject>Adult</subject><subject>Alkaloids - pharmacology</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>ANTIBODIES</subject><subject>Antibodies, Monoclonal</subject><subject>ATP</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>BIOCHEMISTRY</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>CARCINOGENS</subject><subject>Cell biology</subject><subject>CHEMISTRY</subject><subject>CYTOCHEMISTRY</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>Delta cells</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>Enzymes and enzyme inhibitors</subject><subject>ESTERS</subject><subject>flagella</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAMETES</subject><subject>GERM CELLS</subject><subject>Homogenization</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Isoquinolines - pharmacology</subject><subject>ISOTOPES</subject><subject>Kinetics</subject><subject>LIGHT NUCLEI</subject><subject>Male</subject><subject>man</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>motility</subject><subject>NUCLEI</subject><subject>NUCLEOTIDES</subject><subject>ODD-ODD NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>PHORBOL ESTERS</subject><subject>PHOSPHORUS 32</subject><subject>PHOSPHORUS ISOTOPES</subject><subject>PHOSPHORUS-GROUP TRANSFERASES</subject><subject>PHOSPHOTRANSFERASES</subject><subject>Piperazines - pharmacology</subject><subject>Polyclonal antibodies</subject><subject>protein kinase C</subject><subject>Protein Kinase C - analysis</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Protein Kinase C - metabolism</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIOISOTOPES</subject><subject>Rats</subject><subject>Reference Values</subject><subject>requirements</subject><subject>Sperm motility</subject><subject>Sperm Motility - drug effects</subject><subject>SPERMATOZOA</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - enzymology</subject><subject>Spermatozoa - physiology</subject><subject>Sphingosine - pharmacology</subject><subject>Staurosporine</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>TRANSFERASES</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp9kUtv1DAUhS0EKkNhzQZQhASsMvUzthEbNKIUUdQRj7XlOE7ryomntoPov8dhQks3bGzpnu_ee-wDwFME1whycrQbdVoLvkZizQlk98AKQYnqhkp4H6wgxLwWFNOH4FFKlxBCyQQ8AAeYQswQXIGzbQzZurH67MokW20ql6pttMmOuSrlk2nQY_VtZ-PwttqGlFzrbfU1lKOox16fW-91rL6E7LzL14_Bg177ZJ8s9yH4cfzh--akPj37-Gnz_rQ2jMFcM041bq3gEgqCGymJJEw3hAmiO2qJoJbKzlABS1XDDhkMO9Jr3VrS8NaQQ_BuP3c3tYPtTLEbtVe76AYdr1XQTt1VRnehzsNPxSgnuLS_3LeHlJ1KxmVrLkwYR2tyQSiXUhTo9bIjhqvJpqwGl8z83tGGKSnUUIQ5ncGjPWhi-aFo-xsfCKo5JjXHpARXSKg5ptLx_F_7N_ySS9FfLbpORvs-6tG4dDtWMsqaP9yLhZsX_JXvLHrzX0D1k_fZ_sqFfLYnL1MO8dYRYQwjQn4DFYy78A</recordid><startdate>19900901</startdate><enddate>19900901</enddate><creator>Rotem, Ronit</creator><creator>Paz, Gedalia F.</creator><creator>Homonnai, Zvi T.</creator><creator>Kalina, Moshe</creator><creator>Naor, Zvi</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19900901</creationdate><title>Protein Kinase C is Present in Human Sperm: Possible Role in Flagellar Motility</title><author>Rotem, Ronit ; Paz, Gedalia F. ; Homonnai, Zvi T. ; Kalina, Moshe ; Naor, Zvi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-574a2be87908326993935a63583ad4e384e49dc4805a6a0d1c20d3faabe367bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine</topic><topic>560300 - Chemicals Metabolism & Toxicology</topic><topic>Adult</topic><topic>Alkaloids - pharmacology</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>ANTIBODIES</topic><topic>Antibodies, Monoclonal</topic><topic>ATP</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>BIOCHEMISTRY</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>CARCINOGENS</topic><topic>Cell biology</topic><topic>CHEMISTRY</topic><topic>CYTOCHEMISTRY</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>Delta cells</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>Enzymes and enzyme inhibitors</topic><topic>ESTERS</topic><topic>flagella</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAMETES</topic><topic>GERM CELLS</topic><topic>Homogenization</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Isoquinolines - pharmacology</topic><topic>ISOTOPES</topic><topic>Kinetics</topic><topic>LIGHT NUCLEI</topic><topic>Male</topic><topic>man</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>motility</topic><topic>NUCLEI</topic><topic>NUCLEOTIDES</topic><topic>ODD-ODD NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>PHORBOL ESTERS</topic><topic>PHOSPHORUS 32</topic><topic>PHOSPHORUS ISOTOPES</topic><topic>PHOSPHORUS-GROUP TRANSFERASES</topic><topic>PHOSPHOTRANSFERASES</topic><topic>Piperazines - pharmacology</topic><topic>Polyclonal antibodies</topic><topic>protein kinase C</topic><topic>Protein Kinase C - analysis</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Protein Kinase C - metabolism</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIOISOTOPES</topic><topic>Rats</topic><topic>Reference Values</topic><topic>requirements</topic><topic>Sperm motility</topic><topic>Sperm Motility - drug effects</topic><topic>SPERMATOZOA</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - enzymology</topic><topic>Spermatozoa - physiology</topic><topic>Sphingosine - pharmacology</topic><topic>Staurosporine</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>TRANSFERASES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rotem, Ronit</creatorcontrib><creatorcontrib>Paz, Gedalia F.</creatorcontrib><creatorcontrib>Homonnai, Zvi T.</creatorcontrib><creatorcontrib>Kalina, Moshe</creatorcontrib><creatorcontrib>Naor, Zvi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rotem, Ronit</au><au>Paz, Gedalia F.</au><au>Homonnai, Zvi T.</au><au>Kalina, Moshe</au><au>Naor, Zvi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein Kinase C is Present in Human Sperm: Possible Role in Flagellar Motility</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1990-09-01</date><risdate>1990</risdate><volume>87</volume><issue>18</issue><spage>7305</spage><epage>7308</epage><pages>7305-7308</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2402510</pmid><doi>10.1073/pnas.87.18.7305</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1990-09, Vol.87 (18), p.7305-7308 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_19545610 |
| source | JSTOR Archival Journals; PubMed Central |
| subjects | 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine 560300 - Chemicals Metabolism & Toxicology Adult Alkaloids - pharmacology Analytical, structural and metabolic biochemistry ANTIBODIES Antibodies, Monoclonal ATP BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOCHEMISTRY Biological and medical sciences BIOLOGICAL EFFECTS CARCINOGENS Cell biology CHEMISTRY CYTOCHEMISTRY DAYS LIVING RADIOISOTOPES Delta cells ENZYME ACTIVITY ENZYMES Enzymes and enzyme inhibitors ESTERS flagella Fundamental and applied biological sciences. Psychology GAMETES GERM CELLS Homogenization Humans Immunohistochemistry Isoquinolines - pharmacology ISOTOPES Kinetics LIGHT NUCLEI Male man MONOCLONAL ANTIBODIES motility NUCLEI NUCLEOTIDES ODD-ODD NUCLEI ORGANIC COMPOUNDS PHORBOL ESTERS PHOSPHORUS 32 PHOSPHORUS ISOTOPES PHOSPHORUS-GROUP TRANSFERASES PHOSPHOTRANSFERASES Piperazines - pharmacology Polyclonal antibodies protein kinase C Protein Kinase C - analysis Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIOISOTOPES Rats Reference Values requirements Sperm motility Sperm Motility - drug effects SPERMATOZOA Spermatozoa - drug effects Spermatozoa - enzymology Spermatozoa - physiology Sphingosine - pharmacology Staurosporine Tetradecanoylphorbol Acetate - pharmacology TRANSFERASES |
| title | Protein Kinase C is Present in Human Sperm: Possible Role in Flagellar Motility |
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