Molecular cloning and sequence analysis of cDNA encoding δ4-3-ketosteroid 5β-reductase of rat liver

A cDNA clone encoding δ4-3-ketosteroid 5β-reductase was isolated from rat liver cDNA libraries using antibodies specific for the enzyme and oligonucleotides as probes. The cDNA contained 981-base pair open reading frame encoding 327 amino acid residues (M, 37 376) and an unusually long 3′-untranslat...

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Bibliographic Details
Published in:FEBS letters 1991-06, Vol.283 (2), p.215-218
Main Authors: Onishi, Yoshiaki, Noshiro, Mitsuhide, Shimosato, Tsunehiro, Okuda, Kyuichiro
Format: Article
Language:English
Subjects:
Biological and medical sciences
cDNA cloning
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
δ4-3-Ketosteroid 5β-reductase
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Summary:A cDNA clone encoding δ4-3-ketosteroid 5β-reductase was isolated from rat liver cDNA libraries using antibodies specific for the enzyme and oligonucleotides as probes. The cDNA contained 981-base pair open reading frame encoding 327 amino acid residues (M, 37 376) and an unusually long 3′-untranslated region rich in AT sequence in the total length of 3189 base pairs. The predicted amino acid sequence contains th sequences similar to the putative NADPH- and steroid-binding regions.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)80591-P