Fibroblast Growth Factor-Mediated Proliferation of Central Nervous System Precursors Depends on Endogenous Production of Insulin-Like Growth Factor I

• Published in: Proceedings of the National Academy of Sciences - PNAS 1991-03, Vol.88 (6), p.2199-2203
• Main Authors: Drago, John, Murphy, Mark, Carroll, Simon M., Harvey, Richard P., Bartlett, Perry F.
• Format: Article
• Language: English
• Subjects: 550201 - Biochemistry- Tracer Techniques; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; Antibodies; AUTORADIOGRAPHY; AZINES; BASIC BIOLOGICAL SCIENCES; BIOCHEMICAL REACTION KINETICS; Biological and medical sciences; BODY; Cell Division - drug effects; Cell growth; Cell physiology; CELL PROLIFERATION; Cells, Cultured; CENTRAL NERVOUS SYSTEM; CONNECTIVE TISSUE CELLS; Cultured cells; DNA REPLICATION; DNA Replication - drug effects; Embryo, Mammalian; EMBRYOS; Epithelial Cells; EPITHELIUM; Epithelium - drug effects; Epithelium - metabolism; fibroblast growth factor; Fibroblast Growth Factor 2 - pharmacology; FIBROBLASTS; Fundamental and applied biological sciences. Psychology; GROWTH FACTORS; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROGEN COMPOUNDS; INHIBITION; INSULIN; insulin-like growth factor I; Insulin-Like Growth Factor I - biosynthesis; Insulin-Like Growth Factor I - immunology; Insulin-Like Growth Factor I - physiology; KINETICS; MAMMALS; Mesencephalon - cytology; Mesencephalon - drug effects; Mesencephalon - metabolism; MICE; Mice, Inbred CBA; MITOGENS; Molecular and cellular biology; NERVOUS SYSTEM; Neuroepithelial cells; Neurons; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PEPTIDE HORMONES; PROTEINS; PYRIMIDINES; REACTION KINETICS; Receptors; Recombinant Proteins - pharmacology; Responses to growth factors, tumor promotors, other factors; RIBOSIDES; RNA; RNA probes; RODENTS; SOMATIC CELLS; Telencephalon - cytology; Telencephalon - drug effects; Telencephalon - metabolism; THYMIDINE; Thymidine - metabolism; TISSUES; Tritium; TRITIUM COMPOUNDS; VERTEBRATES
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.6.2199

Fibroblast growth factor stimulates proliferation and subsequent differentiation of precursor cells isolated from the neuroepithelium of embryonic day 10 mice in vitro. Here we show that fibroblast growth factor-induced proliferation is dependent on the presence of insulin-like growth factors (IGFs) and that IGF-I is endogenously produced by the neuroepithelial cells. Blocking of endogenous IGF-I activity with anti-IGF-I antibodies results in complete inhibition of fibroblast growth factor-mediated proliferation and in cell death. IGF-I alone acts as a survival agent. These observations correlate with the detection of transcripts for IGF-I and basic fibroblast growth factor in freshly isolated neuroepithelium and are consistent with an autocrine action of these factors in early brain development in vivo.