PAPS‐reductase of Escherichia coli

The DNA of the gene complementing a PAPS‐reductase‐deficient strain of Escherichia coli was sequenced. The N‐terminal amino acid sequence of the purified PAPS‐reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of...

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Bibliographic Details
Published in:FEBS letters 1990-01, Vol.260 (1), p.6-9
Main Authors: Krone, Frank A., Westphal, Goetz, Meyer, Helmut E., Schwenn, Jens D.
Format: Article
Language:English
Subjects:
3'-Phospho-adenylylsulfate reductase
APS, adenylylsulfate
Biological and medical sciences
cys H DNA
Escherichia coli
Fundamental and applied biological sciences. Psychology
Gene product
Genes. Genome
Molecular and cellular biology
Molecular genetics
PAPS, 3'-phospho-adenylyl-sulfate
Phenotypic complementation
PT7 expression
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis
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Summary:The DNA of the gene complementing a PAPS‐reductase‐deficient strain of Escherichia coli was sequenced. The N‐terminal amino acid sequence of the purified PAPS‐reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of M r = 27927. The gene product is functionally active when supplemented with thioredoxin and immunologically related with the wild type enzyme.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)80052-K