Prognostic significance of altered p120ctn expression in bladder cancer

• Published in: BJU international 2008-03, Vol.101 (6), p.746-752
• Main Authors: Neto, Brasil Silva, Smith, Gjanje L., Mandeville, Jessica A., Vanni, Alex J., Wotkowicz, Chad, Rieger‐Christ, Kimberly M., Baumgart, Egbert, Jacobs, Micah A., Cohen, Michael S., Zeheb, Ron, Loda, Massimo, Libertino, John A., Summerhayes, Ian C.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; bladder cancer; Medical sciences; Nephrology. Urinary tract diseases; p120ctn; tissue microarrays; Tumors of the urinary system; Urinary system involvement in other diseases. Miscellaneous; Urinary tract. Prostate gland
• ISSN: 1464-4096; 1464-410X
• DOI: 10.1111/j.1464-410X.2007.07264.x

OBJECTIVE To identify the frequency of change in the expression and localization of p120ctn in bladder tumours and its association with clinical outcomes, and to investigate the potential role of p120ctn in the migratory and invasive behaviour of bladder carcinoma cells. MATERIALS AND METHODS In all, 425 superficial tumour specimens (Ta, Tis and T1) and 305 invasive (T2–T4) tumour specimens from 534 patients were assembled in 10 tissue microarrays. P120ctn immunostaining was scored for intensity and cellular localization and correlated with clinical variables and survival analysis. Knockdown of p120ctn was achieved using small‐interference RNA (siRNA) followed by the assessment of migration and invasion behaviour in standard in vitro assays. RESULTS The expression levels of p120 catenin inversely correlated with pathological tumour stage (P