Evidence for the involvement of CaMKII and AMPK in Ca2+-dependent signaling pathways regulating FA uptake and oxidation in contracting rodent muscle

• Published in: Journal of applied physiology (1985) 2008-05, Vol.104 (5), p.1366-1373
• Main Authors: Raney, Marcella A, Turcotte, Lorraine P
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Caffeine - pharmacology; Calcium Signaling - physiology; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - physiology; Central Nervous System Stimulants - pharmacology; Cyclic AMP-Dependent Protein Kinases - physiology; Extracellular Signal-Regulated MAP Kinases - physiology; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Hindlimb - blood supply; Lactic Acid - blood; Male; Muscle Contraction - drug effects; Muscle Contraction - physiology; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiology; Oxidation-Reduction; Oxygen Consumption - physiology; Palmitates - metabolism; Rats; Rats, Wistar; Regional Blood Flow - physiology
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.01282.2007

Departments of Kinesiology and Biological Sciences, College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, California Submitted 5 December 2007 ; accepted in final form 27 February 2008 Calcium-calmodulin/dependent protein kinase II (CaMKII), AMP-activated protein kinase (AMPK), and extracellular signal-regulated kinase (ERK1/2) have each been implicated in the regulation of substrate metabolism during exercise. The purpose of this study was to determine whether CaMKII is involved in the regulation of FA uptake and oxidation and, if it is involved, whether it does so independently of AMPK and ERK1/2. Rat hindquarters were perfused at rest with ( n = 16) or without ( n = 10) 3 mM caffeine, or during electrical stimulation ( n = 14). For each condition, rats were subdivided and treated with 10 µM of either KN92 or KN93, inactive and active CaMKII inhibitors, respectively. Both caffeine treatment and electrical stimulation significantly increased FA uptake and oxidation. KN93 abolished caffeine-induced FA uptake, decreased contraction-induced FA uptake by 33%, and abolished both caffeine- and contraction-induced FA oxidation ( P < 0.05). Caffeine had no effect on ERK1/2 phosphorylation ( P > 0.05) and increased 2 -AMPK activity by 68% ( P < 0.05). Electrical stimulation increased ERK1/2 phosphorylation and 2 -AMPK activity by 51% and 3.4-fold, respectively ( P < 0.05). KN93 had no effect on caffeine-induced 2 -AMPK activity, ERK1/2 phosphorylation, or contraction-induced ERK1/2 phosphorylation ( P > 0.05). Alternatively, it decreased contraction-induced 2 -AMPK activity by 51% ( P < 0.05), suggesting that CaMKII lies upstream of AMPK. These results demonstrate that regulation of contraction-induced FA uptake and oxidation occurs in part via Ca 2+ -independent activation of ERK1/2 as well as Ca 2+ -dependent activation of CaMKII and AMPK. fatty acid uptake; erk1/2; caffeine; KN93; electrical stimulation Address for reprint requests and other correspondence: Lorraine P. Turcotte, Depts. of Kinesiology and Biological Sciences, College of Letters, Arts, and Sciences, Univ. of Southern California, 3560 Watt Way, PED 107, Los Angeles, CA 90089-0652 (e-mail: turcotte{at}usc.edu )