Determination of S-Adenosylmethionine and S-Adenosylhomocysteine from Human Blood Samples by HPLC-FL

S-adenosylmethionine plays an important role in many biochemical reactions, including human health maintenance and disease prevention. This paper proposes a simplified, rapid, and easy high performance liquid chromatography (HPLC) with fluorimetric detection method of S-adenosylmethionine and its pr...

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Bibliographic Details
Published in:Analytical letters 2008-01, Vol.41 (10), p.1720-1731
Main Authors: Birsan, Camelia, Litescu, Simona C., Cucu, Natalia, Radu, Gabriel L.
Format: Article
Language:English
Subjects:
Analytical chemistry
Blood
Chemistry
Chromatographic methods and physical methods associated with chromatography
ethanoderivatives
Exact sciences and technology
HPLC
Human
Mathematical analysis
Other chromatographic methods
S-adenosylhomocysteine
S-adenosylmethionine
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Summary:S-adenosylmethionine plays an important role in many biochemical reactions, including human health maintenance and disease prevention. This paper proposes a simplified, rapid, and easy high performance liquid chromatography (HPLC) with fluorimetric detection method of S-adenosylmethionine and its product, S-adenosylhomocysteine. Determination of S-adenosylmethionine and S-adenosylhomocysteine was by HPLC with fluorimetric detection by the formation of the fluorescent 1,N 6 -ethanoderivatives of these compounds, which showed a linear concentration range of 310 −6 -310 −8  molL −1 for S-adenosylmethionine and 510 −7 -510 −9 for S-adenosylhomocysteine; the detection limits of the method ranged in the nanomolar domain. The method applied to real plasma samples lead to values of 60.1±19.6 for S-adenosylmethionine and 24±14.1 nmolL −1 for S-adenosylhomocysteine.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710802162236