Molecular Characterization of a Variant of Bacillus anthracis-Specific Phage AP50 with Improved Bacteriolytic Activity

The genome sequence of a Bacillus anthracis-specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2008-11, Vol.74 (21), p.6792-6796
Main Authors: Sozhamannan, Shanmuga, McKinstry, Michael, Lentz, Shannon M, Jalasvuori, Matti, McAfee, Farrell, Smith, Angela, Dabbs, Jason, Ackermann, Hans-W, Bamford, Jaana K.H, Mateczun, Alfred, Read, Timothy D
Format: Article
Language:English
Subjects:
Bacillus
Bacillus anthracis - virology
Bacillus Phages - genetics
Bacteriolysis
Base Sequence
Biological and medical sciences
Colonies
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Gene Order
Genetics and Molecular Biology
Genome, Viral
Genomics
Genotype & phenotype
Gram-positive bacteria
Microbiology
Molecular Sequence Data
Mutation
Mutation, Missense
Point Mutation
Sequence Analysis, DNA
Synteny
Tectiviridae - genetics
Viral Plaque Assay
Virion - ultrastructure
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Summary:The genome sequence of a Bacillus anthracis-specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.01124-08