Messenger ribonucleic acid encoding monocyte chemoattractant protein-1 is expressed by the ovine corpus luteum in response to prostaglandin F2 alpha

To investigate expression of monocyte chemoattractant protein-1 (MCP-1) in the ovine corpus luteum, a partial cDNA was produced by reverse transcription-polymerase chain reaction. This cDNA was 89% identical to that reported for bovine MCP-1 mRNA. In experiment 1, steady-state concentrations of mRNA...

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Bibliographic Details
Published in:Biology of reproduction 1998-01, Vol.58 (1), p.169-174
Main Authors: Haworth, J.D, Rollyson, M.K, Silva, P, McIntush, E.W, Niswender, G.D
Format: Article
Language:English
Subjects:
ADN
ANIMAL PROTEINS
ARN MENSAJERO
ARN MESSAGER
Biological and medical sciences
BREBIS
CELLS
CELLULE
CELULAS
CICLO ESTRAL
COMPLEMENTARY DNA
CORPS JAUNE
CORPUS LUTEUM
CUERPO LUTEO
CYCLE OESTRAL
DNA
EWES
Fundamental and applied biological sciences. Psychology
LOCALIZATION
LUTEAL CELLS
LUTEOLYSIS
Mammalian female genital system
MESSENGER RNA
Morphology. Physiology
OESTROUS CYCLE
OVEJA
PROSTAGLANDINAS
PROSTAGLANDINE
PROSTAGLANDINS
PROTEINAS
PROTEINE
PROTEINS
REGULATION
Vertebrates: reproduction
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Summary:To investigate expression of monocyte chemoattractant protein-1 (MCP-1) in the ovine corpus luteum, a partial cDNA was produced by reverse transcription-polymerase chain reaction. This cDNA was 89% identical to that reported for bovine MCP-1 mRNA. In experiment 1, steady-state concentrations of mRNA encoding MCP-1 were measured in pools of luteal tissue collected on Days 3, 6, 9, 12, and 15 of the estrous cycle (estrus = 0; n = 4/day). There were no differences in mRNA concentrations for MCP-1 among any of the days studied (p = 0.43). In experiment 2, midluteal-phase corpora lutea were collected from ewes at O (untreated), 2, 4, 8, and 16 h after administration of a luteolytic dose of prostaglandin F2 alpha, (PGF2 alpha; n = 4/time point). Concentrations of MCP-1 mRNA were undetectable in untreated controls, were detectable at 2 h post-treatment, had increased 4 and 8 h after administration of PGF2 alpha when compared to those at 2 h (p 0.05), and were decreased 16 h after administration of PGF2 alpha when compared to those at 4 h (p 0.05). In situ hybridization for MCP-1 mRNA combined with immunocytochemical labeling of tissue inhibitor of metalloproteinase-1 (TIMP-1) in large luteal cells was used to determine whether the steroidogenic cells that have PGF2 alpha receptors express MCP-1 mRNA in response to PGF2 alpha. Messenger RNA encoding MCP-1 and TIMP-1 were not colocalized, indicating that MCP-1 was not expressed by large steroidogenic luteal cells during luteolysis
ISSN:0006-3363
1529-7268