Fast induction of high-affinity HCO3- transport in cyanobacteria

• Published in: Plant physiology (Bethesda) 1998-01, Vol.116 (1), p.183-192
• Main Authors: Sultemeyer, D, Klughammer, B, Badger, M.R, Price, G.D
• Format: Article
• Language: English
• Subjects: Acclimatization; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Bacteriology; Badgers; Biological and medical sciences; BIOLOGICAL DIFFERENCES; CANTHARIDIN; CARBON DIOXIDE; CARBON DIOXIDE ENRICHMENT; CARBONATE DEHYDRATASE; CARBONATE DESHYDRATASE; CARBONATO DESHIDRATASA; Cell Biology and Signal Transduction; CELL CULTURE; CELLS; CELLULE; CELULAS; CHLORAMPHENICOL; CLORAMFENICOL; CRECIMIENTO; CROISSANCE; CULTIVO DE CELULAS; CULTURE DE CELLULE; Cyanobacteria; CYANOPHYTA; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; DIOXIDO DE CARBONO; DIOXYDE DE CARBONE; ENRICHISSEMENT EN GAZ CARBONIQUE; ENRIQUECIMIENTO EN GAS CARBONICO; ENZYMIC ACTIVITY; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; FOSFORILACION; Fundamental and applied biological sciences. Psychology; GENETIC REGULATION; GENETIC VARIATION; GENETICA; GENETICS; GENETIQUE; GENISTEIN; GROWTH; Inductive reasoning; INHIBICION; INHIBITION; ION; ION TRANSPORT; IONES; IONS; KINETICS; LIGHT; LUMIERE; LUZ; Metabolism. Enzymes; Microbiology; MONOTERPENOIDE; MONOTERPENOIDES; MONOTERPENOIDS; OKADAIC ACID; PHOSPHORYLATION; Photosynthesis; Plants; Protein synthesis; REGULATION; STAUROSPORINE; STRAIN DIFFERENCES; SYNECHOCOCCUS; TRANSLATION; UPTAKE; VARIACION GENETICA; VARIATION GENETIQUE
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.116.1.183

The induction of a high-affinity state of the CO2-concentration mechanism was investigated in two cyanobacterial species, Synechococcus sp. strain PCC7002 and Synechococcus sp. strain PCC7942. Cells grown at high CO2 concentrations were resuspended in low-CO2 buffer and illuminated in the presence of carbonic anhydrase for 4 to 10 min until the inorganic C compensation point was reached. Thereafter, more than 95% of a high-affinity CO2-concentration mechanism was induced in both species. Mass-spectrometric analysis of CO2 and HCO3- fluxes indicated that only the affinity of HCO3- transport increased during the fast-induction period, whereas maximum transport activities were not affected. The kinetic characteristics of CO2 uptake remained unchanged. Fast induction of high-affinity HCO3- transport was not inhibited by chloramphenicol, cantharidin, or okadaic acid. In contrast, fast induction of high-affinity HCO3- transport did not occur in the presence of K252a, staurosporine, or genistein, which are known inhibitors of protein kinases. These results show that induction of high-affinity HCO3- transport can occur within minutes of exposure to low-inorganic-C conditions and that fast induction may involve posttranslational phosphorylation of existing proteins rather than de novo synthesis of new protein components