Comparison of traditional measurements with macroglycogen and proglycogen analysis of muscle glycogen

Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Traditionally, there have been two methods for measuring total muscle glycogen (Gly tot ), either by acid hydrolysis (AC) or by enzymatic hydrolysis (EZ). As well, it has been determined that rodent muscle...

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Bibliographic Details
Published in:Journal of applied physiology (1985) 1998-03, Vol.84 (3), p.908-913
Main Authors: Adamo, K. B, Graham, T. E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biopsy
Fluorometry
Fundamental and applied biological sciences. Psychology
Glucan 1,4-alpha-Glucosidase
Glycogen - analysis
Humans
Hydrolysis
In Vitro Techniques
Muscle, Skeletal - chemistry
Rats
Reproducibility of Results
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Traditionally, there have been two methods for measuring total muscle glycogen (Gly tot ), either by acid hydrolysis (AC) or by enzymatic hydrolysis (EZ). As well, it has been determined that rodent muscle contains two pools of glycogen, macroglycogen (MG) and proglycogen (PG). This MG/PG determination of Gly tot has never been compared with AC or EZ methods, nor has it been determined whether the two pools exist in human skeletal muscle. A detailed comparison of the three methods was performed by using both rodent and human muscle. It was found that repeated analysis of independent portions of muscle generally gave coefficients of variation of
ISSN:8750-7587
1522-1601
DOI:10.1152/jappl.1998.84.3.908