Detection of PCB77 by Antibody-Coated Competitive Fluorescent Quantitative Immuno-PCR Using Molecular Beacon

A rapid and quantitative technique is urgently needed in detecting toxicological and carcinogenic polychlorinated biphenyls (PCBs) in the environment. In this study, a high sensitive real time fluorescent quantitative immuno-PCR (FQ-IPCR) approach using molecular beacon (MB) was developed to detect...

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Bibliographic Details
Published in:Analytical letters 2009-01, Vol.42 (17), p.2774-2792
Main Authors: Zhuang, Huisheng, Ye, Qiyan, Chen, Hanyu
Format: Article
Language:English
Subjects:
Analytical chemistry
Applied sciences
Beacons
Carcinogens
Chemistry
Detection
Dilution
ELISA
Endocrine disruptors
Exact sciences and technology
FQ-IPCR
Global environmental pollution
Miscellaneous
molecular beacon
PCB77
Pollution
Real time
Reproduction
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Summary:A rapid and quantitative technique is urgently needed in detecting toxicological and carcinogenic polychlorinated biphenyls (PCBs) in the environment. In this study, a high sensitive real time fluorescent quantitative immuno-PCR (FQ-IPCR) approach using molecular beacon (MB) was developed to detect PCB77, which was classified as a human carcinogen. MB-based FQ-IPCR was then performed on serial dilutions of known PCB77 concentrations equivalent to 10-fold dilutions of 10-10 5  fg/mL. A correlation coefficient of 0.997 was identified, and a linear relationship between 10 fg/mL and 100 pg/mL, with y = 0.543x + 21.779, was obtained. Three soil samples were determined by MB-based FQ-IPCR to proof the validity of the method, and the results of which were further confirmed by conventional enzyme-linked immuno sorbent assay (ELISA). Based on sensitivity and reproduction, the MB-based real time FQ-IPCR technique will become a promising monitoring tool for environmental endocrine disruptors.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710903201891