Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique

Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphich DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bo...

Full description

Saved in:
Bibliographic Details
Published in:Applied and Environmental Microbiology 1998-05, Vol.64 (5), p.1954-1957
Main Authors: Deng, M.Q. (University of California-Davis, Davis, CA.), Cliver, D.O
Format: Article
Language:English
Subjects:
Animals
Bacteria
Biological and medical sciences
Brackish
Cattle
CRYPTOSPORIDIUM PARVUM
Cryptosporidium parvum - genetics
Deoxyribonucleic acid
DNA
DNA, Protozoan - analysis
Environmental and Public Health Microbiology
Freshwater
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Marine
Microbiology
Parasites
Polymerase Chain Reaction
Protozoa
Random Amplified Polymorphic DNA Technique
RAPD
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphich DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while five bovine isolates of the same origin were indistinguishable from each other
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.64.5.1954-1957.1998