Escherichia coli Isolates Causing Asymptomatic Bacteriuria in Catheterized and Noncatheterized Individuals Possess Similar Virulence Properties

• Published in: Journal of Clinical Microbiology 2010-07, Vol.48 (7), p.2449-2458
• Main Authors: Watts, Rebecca E, Hancock, Viktoria, Ong, Cheryl-Lynn Y, Vejborg, Rebecca Munk, Mabbett, Amanda N, Totsika, Makrina, Looke, David F, Nimmo, Graeme R, Klemm, Per, Schembri, Mark A
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Bacterial diseases; Bacterial diseases of the urinary system; Bacteriology; Bacteriuria - microbiology; beta-Lactam Resistance - genetics; Biofilms; Biological and medical sciences; Catheters, Indwelling - microbiology; Drug Resistance, Bacterial - genetics; Escherichia coli; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - pathogenicity; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Genes, Bacterial; Human bacterial diseases; Humans; Infectious diseases; Iron - metabolism; Male; Medical sciences; Microbial Sensitivity Tests; Microbiology; Middle Aged; Miscellaneous; Oligonucleotide Array Sequence Analysis; Phylogeny; Urinary Catheterization; Virulence Factors - genetics
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.01611-09

Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli being responsible for >80% of all cases. Asymptomatic bacteriuria (ABU) occurs when bacteria colonize the urinary tract without causing clinical symptoms and can affect both catheterized patients (catheter-associated ABU [CA-ABU]) and noncatheterized patients. Here, we compared the virulence properties of a collection of ABU and CA-ABU nosocomial E. coli isolates in terms of antibiotic resistance, phylogenetic grouping, specific UTI-associated virulence genes, hemagglutination characteristics, and biofilm formation. CA-ABU isolates were similar to ABU isolates with regard to the majority of these characteristics; exceptions were that CA-ABU isolates had a higher prevalence of the polysaccharide capsule marker genes kpsMT II and kpsMT K1, while more ABU strains were capable of mannose-resistant hemagglutination. To examine biofilm growth in detail, we performed a global gene expression analysis with two CA-ABU strains that formed a strong biofilm and that possessed a limited adhesin repertoire. The gene expression profile of the CA-ABU strains during biofilm growth showed considerable overlap with that previously described for the prototype ABU E. coli strain, 83972. This is the first global gene expression analysis of E. coli CA-ABU strains. Overall, our data suggest that nosocomial ABU and CA-ABU E. coli isolates possess similar virulence profiles.