Characterization of Quinolone Resistance in Escherichia coli Strains of Animal Origin from Italy

The aim of this study was to assess the possible circulation of genetic resistance determinants and chromosomal point mutations in quinolone-resistant Escherichia coli isolated from livestock from central italy. forty-nine E. coli isolates were recovered from animals during the surveillance activiti...

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Bibliographic Details
Published in:Journal of chemotherapy (Florence) 2010-06, Vol.22 (3), p.165-168
Main Authors: Forcella, C., Alessiani, A., Perilli, M., Zilli, K., Di Giannatale, E., Amicosante, G.
Format: Article
Language:English
Subjects:
Animals
Anti-Infective Agents - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Blotting, Southern
Cattle - microbiology
Chickens - microbiology
Chromosomes, Bacterial - genetics
Ciprofloxacin - pharmacology
Drug Resistance, Bacterial
Escherichia coli - drug effects
Escherichia coli - isolation & purification
Escherichia coli Proteins - genetics
Italy
Medical sciences
Nalidixic Acid - pharmacology
Pharmacology. Drug treatments
Plasmids - genetics
Polymerase Chain Reaction
Quinolone
Rabbits - microbiology
resistance
Sheep - microbiology
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Summary:The aim of this study was to assess the possible circulation of genetic resistance determinants and chromosomal point mutations in quinolone-resistant Escherichia coli isolated from livestock from central italy. forty-nine E. coli isolates were recovered from animals during the surveillance activities of the Istituto Zooprofilattico Abruzzo e Molise (IZSA&M), Italy, over 2 years. the plasmid resistance determinants and point mutations in DNA gyrase and topoisomerase IV were characterized by PCR and DNA sequencing. Of the 49 E. coli isolates, 34 were resistant to nalidixic acid, 4 to ciprofloxacin and 11 to nalidixic acid, ciprofloxacin and enrofloxacin. Chromosomal point mutations were found in gyrA gene (Ser83Leu and Asp87Asn) and gyr B (Gln434His, Lys444Arg and Gly435Val). We also report the simultaneous presence of qnrS1 quinolone resistance determinant dfrA1-aadA22 gene cassettes and amino acid substitution Ser83Leu in the gyrA gene in an E. coli strain resistant only to nalidixic acid.
ISSN:1120-009X
1973-9478
DOI:10.1179/joc.2010.22.3.165