A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network

SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (6), p.1302-1305
Main Authors: TANAKA, Niina, SAITOH, Hisato
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Assaying
Assembly
Biochemistry
Biological and medical sciences
Biotechnology
chromatin
Fundamental and applied biological sciences. Psychology
Gain
Genes
histone methyltransferase
Humans
post-translational modification
Protein Binding
protein interaction
Protein Interaction Mapping - methods
Protein Methyltransferases - chemistry
Protein Methyltransferases - metabolism
Proteins
Real time
Repressor Proteins
Screening
SUMO
SUMO-1 Protein - metabolism
Time Factors
Transcription Factors - chemistry
Transcription Factors - metabolism
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Summary:SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.
ISSN:0916-8451
1347-6947
1347-6947
DOI:10.1271/bbb.100082