Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay

Purpose: The purpose of this study was to find an in vitro method for determining the cytotoxicity of boronated drugs as well as their potential suitability for neutron capture therapy. Materials and methods: The survival of human melanoma cells has been determined by a colony assay and the sulforho...

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Bibliographic Details
Published in:International journal of radiation biology 1998, Vol.73 (6), p.679-690
Main Authors: Wittig, A, Sauerwein, W, Pöller, F, Fuhrmann, C, Hideghéty, K, Streffer, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Boron Neutron Capture Therapy
Carcinogenesis, carcinogens and anticarcinogens
Cell Survival - radiation effects
Dose-Response Relationship, Radiation
Fast Neutrons
Fluorescent Dyes
Humans
Medical sciences
Melanoma - pathology
Melanoma - radiotherapy
Physical agents
Rhodamines
Tumor Cells, Cultured
Tumor Stem Cell Assay
Tumors
X-Rays
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Summary:Purpose: The purpose of this study was to find an in vitro method for determining the cytotoxicity of boronated drugs as well as their potential suitability for neutron capture therapy. Materials and methods: The survival of human melanoma cells has been determined by a colony assay and the sulforhodamine-B assay after X-irradiation and irradiation with fast d(14) Beneutrons using the boronated compound borocaptate sodium (BSH). The cytotoxic effects of BSH have been studied using both methods. Results: Under well-defined experimental conditions, and after a sufficient amount of time for the expression of radiation damage, the results of the sulforhodamine-B assay are qualitatively comparable with the results of the colony assay. Conclusion: The sulforhodamine-B assay is suitable for the screening of compounds for potential use in neutron capture therapy because it is a fast and efficient method that is reproducible and technically advantageous.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530098141933