Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (10), p.2011-2015
• Main Authors: YAN, Hanxu, TIAN, Huaize, KINJO, Tomoya, HAMASAKI, Takeki, TOMIMATSU, Kosuke, NAKAMICHI, Noboru, TERUYA, Kiichiro, KABAYAMA, Shigeru, SHIRAHATA, Sanetaka
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Caenorhabditis elegans; Caenorhabditis elegans - drug effects; Caenorhabditis elegans - metabolism; Caenorhabditis elegans - physiology; Culture Media - chemistry; Culture Media - pharmacology; Electrolysis; electrolyzed reduced water; Fundamental and applied biological sciences. Psychology; lifespan; Longevity - drug effects; Nematoda; reactive oxygen species; Reactive Oxygen Species - metabolism; Water - chemistry; Water - pharmacology
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.100250

Electrolyzed reduced water (ERW) has attracted much attention because of its therapeutic effects. In the present study, a new culture medium, which we designated Water medium, was developed to elucidate the effects of ERW on the lifespan of Caenorhabditis elegans. Wild-type C. elegans had a significantly shorter lifespan in Water medium than in conventional S medium. However, worms cultured in ERW-Water medium exhibited a significantly extended lifespan (from 11% to 41%) compared with worms cultured in ultrapure water-Water medium. There was no difference between the lifespans of worms cultured in ERW-S medium and ultrapure water-S medium. Nematodes cultured in ultrapure water-Water medium showed significantly higher levels of reactive oxygen species than those cultured in ultrapure water-S medium. Moreover, ERW-Water medium significantly reduced the ROS accumulation induced in the worms by paraquat, suggesting that ERW-Water medium extends the longevity of nematodes at least partly by scavenging ROS.