mathFISH, a Web Tool That Uses Thermodynamics-Based Mathematical Models for In Silico Evaluation of Oligonucleotide Probes for Fluorescence In Situ Hybridization

Mathematical models of RNA-targeted fluorescence in situ hybridization (FISH) for perfectly matched and mismatched probe/target pairs are organized and automated in web-based mathFISH (http://mathfish.cee.wisc.edu). Offering the users up-to-date knowledge of hybridization thermodynamics within a the...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2011-02, Vol.77 (3), p.1118-1122
Main Authors: Yilmaz, L. Safak, Parnerkar, Shreyas, Noguera, Daniel R
Format: Article
Language:English
Subjects:
Algorithms
Base Pair Mismatch
Biological and medical sciences
Fluorescence in situ hybridization
Fundamental and applied biological sciences. Psychology
In Situ Hybridization, Fluorescence - methods
Internet
Mathematical models
Methods
Microbiology
Models, Theoretical
Oligonucleotide Probes - analysis
Oligonucleotide Probes - genetics
Protein synthesis
RNA - genetics
Software
Thermodynamics
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Summary:Mathematical models of RNA-targeted fluorescence in situ hybridization (FISH) for perfectly matched and mismatched probe/target pairs are organized and automated in web-based mathFISH (http://mathfish.cee.wisc.edu). Offering the users up-to-date knowledge of hybridization thermodynamics within a theoretical framework, mathFISH is expected to maximize the probability of success during oligonucleotide probe design.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.01733-10