Differential inspiratory timing is genetically linked to mouse chromosome 3

• Published in: Journal of applied physiology (1985) 1998-07, Vol.85 (1), p.360-365
• Main Authors: Tankersley, C. G, DiSilvestre, D. A, Jedlicka, A. E, Wilkins, H. M, Zhang, L
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cardiorespiratory control. Arterial mecano- and chemoreceptor; Chromosomes - genetics; Chromosomes - physiology; DNA - genetics; DNA - isolation & purification; Female; Fundamental and applied biological sciences. Psychology; Genes; Genetic Linkage - genetics; Genetics; Male; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Phenotype; Polymerase Chain Reaction; Quantitative Trait, Heritable; Respiratory Mechanics - genetics; Respiratory Mechanics - physiology; Respiratory system; Rodents; Vertebrates: respiratory system
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.1998.85.1.360

Departments of Anesthesiology and Environmental Health Sciences, The Johns Hopkins University, Baltimore, Maryland 21205 Genetic control of differential inspiratory timing (T I ) at baseline has been previously demonstrated among inbred mouse strains. The inheritance pattern for T I between C3H/HeJ (C3; 188 ± 3 ms) and C57BL/6J (B6; 111 ± 2 ms) progenitors was consistent with a two-gene model. By using the strain distribution pattern for recombinant inbred strains derived from C3 and B6 progenitors, 100% concordance was established between T I phenotypes and DNA markers on mouse chromosome 3. This genotype-phenotype hypothesis was tested by typing 52 B6C3F 2 (F 2 ) progeny by using simple sequence repeat DNA markers ( n  = 21) polymorphic between C3 and B6 strains on mouse chromosome 3. Linkage analysis compared marker genotypes to baseline ventilatory phenotypes by computing log-likelihood values. A putative quantitative trait locus located in proximity to D3Mit119 was significantly associated with baseline T I phenotypes. At the peak (log-likelihood = 3.3), the putative quantitative trait locus determined 25% of the phenotypic variance in T I among F 2 progeny. In conclusion, this genetic model of ventilatory characteristics demonstrated an important linkage between differential baseline T I and a candidate genomic region on mouse chromosome 3. control of breathing; linkage analysis; C3H/HeJ; C57BL/6J; BXH recombinant inbred strains