Ac magnetic susceptibility study of in vivo nanoparticle biodistribution

• Published in: Journal of physics. D, Applied physics Applied physics, 2011-06, Vol.44 (25), p.255002
• Main Authors: Gutiérrez, L, Mejías, R, Barber, D F, Veintemillas-Verdaguer, S, Serna, C J, Lázaro, F J, Morales, M P
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Materials science; Methods. Procedures. Technologies; Nanopowders; Nanoscale materials and structures: fabrication and characterization; Others; Physics; Various methods and equipments
• ISSN: 0022-3727; 1361-6463
• DOI: 10.1088/0022-3727/44/25/255002

We analysed magnetic nanoparticle biodistribution, before and after cytokine conjugation, in a mouse model by ac susceptibility measurements of the corresponding resected tissues. Mice received repeated intravenous injections of nanoparticle suspension for two weeks and they were euthanized 1 h after the last injection. In general, only 10% of the total injected nanoparticles after multiple exposures were found in tissues. The rest of the particles may probably be metabolized or excreted by the organism. Our findings indicate that the adsorption of interferon to DMSA-coated magnetic nanoparticles changes their biodistribution, reducing the presence of nanoparticles in lungs and therefore their possible toxicity. The specific targeting of the particles to tumour tissues by the use of an external magnetic field has also been studied. Magnetic nanoparticles were observed by transmission electron microscopy in the targeted tissue and quantified by ac magnetic susceptibility.