Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies

Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslationa...

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Bibliographic Details
Published in:Journal of Bacteriology 2011-09, Vol.193 (18), p.4984-4987
Main Authors: Dinh, Thuy, Bernhardt, Thomas G
Format: Article
Language:English
Subjects:
Bacterial proteins
Bacteriology
Biological and medical sciences
Fluorescence
Fundamental and applied biological sciences. Psychology
green fluorescent protein
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Microbial Cell Biology
Microbiology
Miscellaneous
Molecular Biology - methods
Periplasm - chemistry
Periplasmic Proteins - analysis
Periplasmic Proteins - genetics
Protein folding
Recombinant Fusion Proteins - analysis
Recombinant Fusion Proteins - genetics
Staining and Labeling - methods
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Summary:Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/JB.00315-11