F33:A–:B– and F2:A–:B– Plasmids Mediate Dissemination of rmtB-blaCTX-M-9 Group Genes and rmtB-qepA in Enterobacteriaceae Isolates from Pets in China

• Published in: Antimicrobial Agents and Chemotherapy 2011-10, Vol.55 (10), p.4926-4929
• Main Authors: Deng, Yuting, He, Liangying, Chen, Sheng, Zheng, Hongqing, Zeng, Zhenling, Liu, Yahong, Sun, Yan, Ma, Junying, Chen, Zhangliu, Liu, Jian-Hua
• Format: Article
• Language: English
• Subjects: Aminoglycosides; Aminoglycosides - pharmacology; Animals; Anti-Bacterial Agents; Anti-Bacterial Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial Proteins; Bacterial Proteins - genetics; beta-Lactamases; beta-Lactamases - genetics; Biological and medical sciences; Cats; China; Dogs; drug effects; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae; Enterobacteriaceae - drug effects; Enterobacteriaceae - genetics; Enterobacteriaceae - isolation & purification; Epidemiology and Surveillance; Escherichia coli Proteins; Escherichia coli Proteins - genetics; Gene Transfer, Horizontal; genes; genetics; isolation & purification; Medical sciences; Methyltransferases; Methyltransferases - genetics; Microbial Sensitivity Tests; microbiology; Molecular Sequence Data; Pets; Pets - microbiology; pharmacology; Pharmacology. Drug treatments; Plasmids; Plasmids - genetics; ribosomal RNA; RNA, Ribosomal, 16S; RNA, Ribosomal, 16S - genetics
• ISSN: 0066-4804; 1098-6596
• DOI: 10.1128/aac.00133-11

This study investigated the prevalence of 16S rRNA methylase genes in 267 Enterobacteriaceae isolates collected from pets. The rmtB gene was detected in 69 isolates, most of which were clonally unrelated. The coexistence of the rmtB gene with the blaCTX-M-9 group genes and/or qepA within the same IncFII replicons was commonly detected. The two dominant types of IncF plasmids, F2:A–:B–, carrying rmtB-qepA, and F33:A–:B–, carrying the rmtB-blaCTX-M-9 group genes (and especially blaCTX-M-65), shared restriction patterns within each incompatibility group.