Reducing effects of garlic constituents on DNA adduct formation in human lymphocytes in vitro

A water extract of raw garlic (RGE) and two organosulfur compounds, diallyl sulfide and S-allylcysteine (SAC), were evaluated for their relative effectiveness in reducing benzo[a]pyrene (BaP)-DNA adduct formation in stimulated human peripheral blood lymphocytes in vitro. In replicate experiments, RG...

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Bibliographic Details
Published in:Nutrition and cancer 1997-01, Vol.27 (2), p.177-185
Main Authors: Hageman, Geja J., van Herwijnen, Marcel H. M., Schilderman, Pauline A. E. L., Rhijnsburger, Els H., Moonen, Edwin J. C., Kleinjans, Jos C. S.
Format: Article
Language:English
Subjects:
Allyl Compounds
Anticarcinogenic Agents - pharmacology
Benzo(a)pyrene - metabolism
Biological and medical sciences
Cell Division
Cell Survival
Cells, Cultured
Cysteine - analogs & derivatives
Cysteine - pharmacology
Disulfides - pharmacology
DNA Adducts - blood
Free Radical Scavengers
Garlic
General and cellular metabolism. Vitamins
Humans
Lymphocytes - drug effects
Lymphocytes - metabolism
Medical sciences
Pharmacology. Drug treatments
Plant Extracts - pharmacology
Plants, Medicinal
Reactive Oxygen Species - metabolism
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Summary:A water extract of raw garlic (RGE) and two organosulfur compounds, diallyl sulfide and S-allylcysteine (SAC), were evaluated for their relative effectiveness in reducing benzo[a]pyrene (BaP)-DNA adduct formation in stimulated human peripheral blood lymphocytes in vitro. In replicate experiments, RGE significantly inhibited BaP-DNA adduct formation at concentrations of 0.001, 0.01, and 0.1 mg/ml. SAC also significantly decreased BaP-DNA adduct formation at concentrations of 0.01 and 0.1 mg/ml. For diallyl sulfide, no significant reduction in BaP-DNA adduct formation was found. BaP-DNA adduct formation was not associated with cell viability or proliferation of peripheral blood lymphocytes after the various treatments. No clear scavenging activity was detected for the garlic constituents. Aryl hydrocarbon hydroxylase activity was not decreased, nor was formation of sulfate and glucuronide conjugates of 3-hydroxy-BaP increased in the presence of RGE and SAC, indicating that increased glutathione S-transferase activity or a more efficient repair of BaP-DNA adducts may explain the observed effects. In addition, reactive oxygen species-induced 8-oxodeoxyguanosine in DNA was reduced in the presence of SAC. It is concluded that raw garlic and SAC may be useful in the prevention of BaP-associated tu-morigenesis and that further evaluation of their preventive potential in humans at risk appears feasible.
ISSN:0163-5581
1532-7914
DOI:10.1080/01635589709514522