Validation of a commercial ELISA for the analysis of the insecticide dinotefuran in a variety of analytically challenging vegetables

The main objective of this paper was to assess the suitability of an enzyme-linked immunosorbent assay (ELISA) as a rapid and simple screening method for the detection of neonicotinoid insecticide dinotefuran residues in various vegetable samples including carrot, cabbage, green pepper, leek, Japane...

Full description

Saved in:
Bibliographic Details
Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2012-07, Vol.29 (7), p.1067-1073
Main Authors: Watanabe, E, Kobara, Y, Miyake, S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Brassica
Brassica rapa subsp. nipposinica var. perviridis
cabbage
carrots
Chromatography, High Pressure Liquid
Daucus
detection limit
dinotefuran
Enzyme-Linked Immunosorbent Assay
Enzymes
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Guanidines - analysis
high performance liquid chromatography
immunoassays
Insecticides
Insecticides - analysis
leeks
Methanol
Neonicotinoids
Nitro Compounds - analysis
pesticide residues
pretreatment
screening
screening - ELISA
screening - immunoassays
spinach
Spinacia oleracea
Studies
sweet peppers
Vegetables
Vegetables - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The main objective of this paper was to assess the suitability of an enzyme-linked immunosorbent assay (ELISA) as a rapid and simple screening method for the detection of neonicotinoid insecticide dinotefuran residues in various vegetable samples including carrot, cabbage, green pepper, leek, Japanese mustard spinach (komatsuna), and spinach. Simple dilution of crude sample extracts was effective to circumvent or reduce matrix interference derived from samples. Consequently, ELISA was useful to determine dinotefuran accurately and directly, only requiring methanol extraction and dilution with water. The theoretically estimated limit of detection of dinotefuran for tested samples ranged from 0.06 to 0.12 mg kg⁻¹. The ELISA gave recovery values close to 100% for all samples except komatsuna, which contained lower concentration levels, and the results of the ELISA agreed well with those obtained with conventional HPLC (r > 0.99). Results show that the ELISA evaluated in this study is suitable for the rapid and simple screening method of dinotefuran residue in agricultural samples even without sample pre-treatment.
ISSN:1944-0057
1944-0049
1944-0057
DOI:10.1080/19440049.2012.681400