Coulometric Electrochemical Detection of Phospholipid Hydroperoxides by High-performance Liquid Chromatography

A technique for the sensitive and convenient determination of phosphatidylcholine hydroperoxides was developed that uses coulometric electrochemical detection in a high-performance liquid chromatographic analysis. The detection limit for phosphatidylcholine hydroperoxides by this method was 20 pmol...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1997-01, Vol.61 (1), p.191-193
Main Authors: Arai, Hirofumi, Mohri, Satoshi, Suzuki, Tetsuya, Takama, Kozo, Terao, Junji
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
coulometric electrochemical detection
Fundamental and applied biological sciences. Psychology
Glycerolipids, phospholipids
HPLC
Lipids
Other biological molecules
phospholipid hydroperoxide
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Summary:A technique for the sensitive and convenient determination of phosphatidylcholine hydroperoxides was developed that uses coulometric electrochemical detection in a high-performance liquid chromatographic analysis. The detection limit for phosphatidylcholine hydroperoxides by this method was 20 pmol at a signal-to-noise ratio of 3. The glutathione-dependent phospholipid hydroperoxide-reducing activity of fresh human plasma was ascertained by this method.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.61.191