Coulometric Electrochemical Detection of Phospholipid Hydroperoxides by High-performance Liquid Chromatography

A technique for the sensitive and convenient determination of phosphatidylcholine hydroperoxides was developed that uses coulometric electrochemical detection in a high-performance liquid chromatographic analysis. The detection limit for phosphatidylcholine hydroperoxides by this method was 20 pmol...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1997-01, Vol.61 (1), p.191-193
Main Authors: Arai, Hirofumi, Mohri, Satoshi, Suzuki, Tetsuya, Takama, Kozo, Terao, Junji
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
coulometric electrochemical detection
Fundamental and applied biological sciences. Psychology
Glycerolipids, phospholipids
HPLC
Lipids
Other biological molecules
phospholipid hydroperoxide
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container_end_page 193
container_issue 1
container_start_page 191
container_title Bioscience, biotechnology, and biochemistry
container_volume 61
creator Arai, Hirofumi
Mohri, Satoshi
Suzuki, Tetsuya
Takama, Kozo
Terao, Junji
description A technique for the sensitive and convenient determination of phosphatidylcholine hydroperoxides was developed that uses coulometric electrochemical detection in a high-performance liquid chromatographic analysis. The detection limit for phosphatidylcholine hydroperoxides by this method was 20 pmol at a signal-to-noise ratio of 3. The glutathione-dependent phospholipid hydroperoxide-reducing activity of fresh human plasma was ascertained by this method.
doi_str_mv 10.1271/bbb.61.191
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ispartof Bioscience, biotechnology, and biochemistry, 1997-01, Vol.61 (1), p.191-193
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source Electronic Journals Library; Oxford Journals Online
subjects Analytical, structural and metabolic biochemistry
Biological and medical sciences
coulometric electrochemical detection
Fundamental and applied biological sciences. Psychology
Glycerolipids, phospholipids
HPLC
Lipids
Other biological molecules
phospholipid hydroperoxide
title Coulometric Electrochemical Detection of Phospholipid Hydroperoxides by High-performance Liquid Chromatography
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