Tapetum-specific expression of the Osg6B promoter-beta-glucuronidase gene in transgenic rice

The promoter of an anther tapetum-specific gene Osg6B, was fused to a beta-glucuronidase (GUS) gene and introduced into rice by Agrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate...

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Bibliographic Details
Published in:Plant cell reports 1997-03, Vol.16 (6), p.363-367
Main Authors: Yokoi, S, Tsuchiya, T, Toriyama, K, Hinata, K
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
anther culture
anthers
beta-glucuronidase
Biological and medical sciences
Biotechnology
culture media
enzyme activity
Fundamental and applied biological sciences. Psychology
gene expression
genes
Genetic engineering
Genetic technics
genetic transformation
histochemistry
localization
Methods. Procedures. Technologies
Modification of gene expression level
Oryza sativa
promoter regions
transgenic plants
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Summary:The promoter of an anther tapetum-specific gene Osg6B, was fused to a beta-glucuronidase (GUS) gene and introduced into rice by Agrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate microspore stage (7 days before anthesis) to the tricellular pollen stage (3 days before anthesis). This is the first demonstration of an anther-specific promoter directing tapetum-specific expression in rice.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050240