Albumin nanoparticles with predictable size by desolvation procedure

Nanoparticles prepared of human serum albumin (HSA) represent promising carriers for drug delivery. Desolvating procedure is a common method to obtain protein-based particles. However process parameters have a great influence on size, size distribution and particle yield of the resulting formulation...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microencapsulation 2012-03, Vol.29 (2), p.138-146
Main Authors: von Storp, B., Engel, A., Boeker, A., Ploeger, M., Langer, K.
Format: Article
Language:English
Subjects:
Biological and medical sciences
desolvation procedure
Drug Delivery Systems
General pharmacology
human serum albumin
Medical sciences
nanoparticles
Nanoparticles - administration & dosage
Particle Size
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
polydispersity
Serum Albumin - administration & dosage
Solubility
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nanoparticles prepared of human serum albumin (HSA) represent promising carriers for drug delivery. Desolvating procedure is a common method to obtain protein-based particles. However process parameters have a great influence on size, size distribution and particle yield of the resulting formulation. Therefore, it is crucial to investigate their effects on particle preparation. This study is focused on the influence of different desolvating agents on the resulting particle characteristics and cytotoxicity in cell culture. Another aspect of the study was the influence of different stirring rates, pre-stirring of the HSA solution and the continuous addition of desolvating agent during the preparation process. The results indicate that it is possible to predict the particle size depending on dielectric constant of the desolvation medium. Remarkably, combination of methanol and ethanol used as desolvating agent was able to produce very small spherical HSA nanoparticles in a size range between 50 and 80 nm.
ISSN:0265-2048
1464-5246
DOI:10.3109/02652048.2011.635218