Determination of Sodium Acetate in Antisense Oligonucleotides by Capillary Zone Electrophoresis

The determination of sodium acetate in anti sense oligonucleotides purified by reverse phase chromatography in acetate buffer is important in both impurity profiling and drug concentration assay. A method has been developed for the quantitation of sodium acetate in a 20-mer phosphorothioate oligonuc...

Full description

Saved in:
Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 1997-01, Vol.20 (8), p.1185-1195
Main Authors: Chen, Danhua, Klopchin, Pat, Parsons, Jodi, Srivatsa, G. Susan
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The determination of sodium acetate in anti sense oligonucleotides purified by reverse phase chromatography in acetate buffer is important in both impurity profiling and drug concentration assay. A method has been developed for the quantitation of sodium acetate in a 20-mer phosphorothioate oligonucleotide drug substance employing capillary zone electrophoresis. Chloride was included as an internal standard to increase accuracy and precision of the measurement. Interference due to oligonucleotide matrix was eliminated by filtering the olignucleotide solution through a Centricon SR-3 column. The linear dynamic range for the method is 0.06-240 μg/mL. The electrophoretic separation requires only 6 min. The method has bee-a demonstrated to be simple and reliable for the quantitation of :Acetate salts in oligonucleotide drug substances.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826079708010968