Fatty Acid Esters of Sitosterol 3β-Glucoside from Soybeans and Tempe (Fermented Soybeans) as Antiproliferative Substances

• Published in: Journal of Clinical Biochemistry and Nutrition 1997, Vol.22(3), pp.139-147
• Main Authors: KIRIAKIDIS, Serafim, STATHI, Stavroula, JHA, Hem Chandra, HARTMANN, Rudolf, EGGE, Heinz
• Format: Article
• Language: English
• Subjects: antitumor activity; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; fermented soybean; Foods and miscellaneous; General pharmacology; Medical sciences; mouse myeloma cells X63-Ag8.653; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; sitosterol; sitosterol glucoside; sitosterol-3-O-β-D-(6′-0-linoleoyl)-glucopyranoside; sitosterol-3-O-β-D-(6′-O-palmitoyl)-glucopyranoside; sitosterol-3-O-β-D-(6′-O-stearoyl)-glucopyranoside; Tumors
• ISSN: 0912-0009; 1880-5086
• DOI: 10.3164/jcbn.22.139

In the present study we isolated and identified two mixtures of fatty acid esters of β-sitosterol glucoside from soybeans and its fermented product, tempe. The mixtures of glucolipids occurring in soybeans and tempe are unseparable and possess the following structures: sitosterol-3-O-β-D-(6′-O-palmitoyl/-linoleoyl)- and sitosterol-3-O-β-D-(6′-O-palmitoyl/-stearoyl)-glucopyranoside, respectively. Since tempe constituents show antitumor activity, we tested these glucolipids against mouse myeloma cells (tumor cells). The tempe glucolipid mixture showed higher inhibition (95.3%) at the 50μg/ml concentration than the soybean one. The maximum activity of the soybean glucolipid mixture was (96%) at 100μg/ml.