Formation of a Novel Odd Chain Polyunsaturated Fatty Acid, 5,8,11,14,17-cis-Nonadecapentaenoic Acid, by an EPA-Producing Aquatic Fungus, Saprolegnia sp. 28YTF-1

An aquatic filamentous fungus, Saprolegnia sp. 28YTF-1, was found to accumulate odd chain polyunsaturated fatty acids (PUFAs) of 17 to 19 carbon chain length, when grown with fatty acids with 13, 15, or 17 carbons. A novel ω2 PUFA identified as 5,8,11,14,17-cis-nonadecapentaenoic acid (19:5ω2) was d...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (10), p.1963-1965
Main Authors: Shirasaka, Norifumi, Yokochi, Toshihiro, Shimizu, Sakayu
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Freshwater
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Mission oriented research
Mycology
Physiology and metabolism
Saprolegnia
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Summary:An aquatic filamentous fungus, Saprolegnia sp. 28YTF-1, was found to accumulate odd chain polyunsaturated fatty acids (PUFAs) of 17 to 19 carbon chain length, when grown with fatty acids with 13, 15, or 17 carbons. A novel ω2 PUFA identified as 5,8,11,14,17-cis-nonadecapentaenoic acid (19:5ω2) was detected as one of the major PUFAs, together with 5,8,11,14-cis-nona-decatetraenoic acid (19:4ω5), in both nonpolar and polar lipids from the mycelia. The biosynthetic route to 19:5ω2 was presumed to mimic the ω6 route to arachidonic acid (20:4ω6) and ω3 de-saturation of 20:4ω6 to 5,8,11,14,17 -cis-eicosapentaenoic acid (20:5ω3), as follows: 15:0→17:0→17:1→17:2ω5→17:3ω5→19:4ω5→19:5ω2.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.59.1963