Evidence for Lowered Induction of Nuclear Factor Kappa B in Activated Human T Lymphocytes during Aging

Transcription factor NFκB (nuclear factor κB) is induced in T lymphocytes from young individuals following activation with a variety of stimuli including anti-CD3, phorbol myristate acetate (PMA), and tumor necrosis factor-α (TNF-α). In contrast, activated T lymphocytes from older individuals show a...

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Published in:Gerontology (Basel) 1996, Vol.42 (3), p.137-146
Main Authors: Trebilcock, Gina Uken, Ponnappan, Usha
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Ageing, cell death
Aging - immunology
Biological and medical sciences
Carcinogens - pharmacology
CD3 Complex - immunology
Cell physiology
Experimental Section
Female
Fundamental and applied biological sciences. Psychology
Humans
Lymphocyte Activation - drug effects
Male
Molecular and cellular biology
NF-kappa B - biosynthesis
Oligonucleotide Probes
T-Lymphocytes - immunology
Tetradecanoylphorbol Acetate - pharmacology
Tumor Necrosis Factor-alpha - pharmacology
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container_issue 3
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container_title Gerontology (Basel)
container_volume 42
creator Trebilcock, Gina Uken
Ponnappan, Usha
description Transcription factor NFκB (nuclear factor κB) is induced in T lymphocytes from young individuals following activation with a variety of stimuli including anti-CD3, phorbol myristate acetate (PMA), and tumor necrosis factor-α (TNF-α). In contrast, activated T lymphocytes from older individuals show a significant reduction in the induction of NFκB in response to the same stimuli. The age-related decline in induction of NFκB could not be attributed to alteration in the composition of subunits, p50 and p65 were found to be the predominant subunits of induced NFκB in T cells from young as well as elderly donors. Furthermore, similar levels of NFκB were found in the cytosols of unactivated T cells from both young and elderly donors suggesting that precursor levels of NFκB remain unaltered during aging. These results suggest that an age-associated decline in the induction of NFκB in activated T cells from elderly individuals may be attributable to altered regulation of the inhibitor, IκB, and may play an important role in immune dysregulation accompanying aging.
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ispartof Gerontology (Basel), 1996, Vol.42 (3), p.137-146
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subjects Adult
Aged
Aged, 80 and over
Ageing, cell death
Aging - immunology
Biological and medical sciences
Carcinogens - pharmacology
CD3 Complex - immunology
Cell physiology
Experimental Section
Female
Fundamental and applied biological sciences. Psychology
Humans
Lymphocyte Activation - drug effects
Male
Molecular and cellular biology
NF-kappa B - biosynthesis
Oligonucleotide Probes
T-Lymphocytes - immunology
Tetradecanoylphorbol Acetate - pharmacology
Tumor Necrosis Factor-alpha - pharmacology
title Evidence for Lowered Induction of Nuclear Factor Kappa B in Activated Human T Lymphocytes during Aging
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