1L-myo-inositol 1-phosphate synthase from Arabidopsis thaliana

A recombinant phage containing an Arabidopsis thaliana cDNA sequence encoding a protein with 1L-myo-inositol 1-phosphate synthase (EC 5.5.1.4) activity has been isolated and used for transcriptional and translational studies. The identification of the recombinant phage relied on the observations tha...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1995-02, Vol.107 (2), p.613-619
Main Authors: Margaret Dean Johnson, Sussex, Ian M.
Format: Article
Language:English
Subjects:
actividad enzimatica
activite enzymatique
arabidopsis thaliana
Biological and medical sciences
Biosynthesis
clone
clones
code genetique
codigo genetico
Complementary DNA
DNA
Enzymes
enzymic activity
expresion genica
expression des genes
fosfatos
Fundamental and applied biological sciences. Psychology
Gels
gene
gene expression
genes
genetic code
genetica
genetics
genetique
inositol
Inositols
isolation techniques
ligasas
ligase
ligases
Metabolism
Metabolism. Physicochemical requirements
oxidoreductases
oxidorreductasas
oxydoreductase
phosphate
phosphates
Plant physiology and development
Plant-Microbe and Plant-Insect Interactions
Plasmids
RNA
Seedlings
technique de l' isolement
tecnicas de aislamiento
Yeasts
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A recombinant phage containing an Arabidopsis thaliana cDNA sequence encoding a protein with 1L-myo-inositol 1-phosphate synthase (EC 5.5.1.4) activity has been isolated and used for transcriptional and translational studies. The identification of the recombinant phage relied on the observations that (a) the clone complements a mutation in the structural gene for 1L-myo-inositol 1-phosphate synthase in the yeast Saccharomyces cerevisiae, (b) the in vitro synthesized polypeptide enzymatically converts glucose 6-phosphate into inositol 1-phosphate, (c) in vitro transcription and translation of this cDNA sequence produces a polypeptide that is recognized by anti-yeast myo-inositol 1-phosphate synthase antiserum, and (d) inositol regulates the expression of the corresponding gene in Arabidopsis.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.107.2.613