Biosynthesis of cyclic beta-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii

Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic beta-(1.2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobilit...

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Bibliographic Details
Published in:Archives of microbiology 1995-06, Vol.163 (6), p.454-462
Main Authors: Castro, O.A, Zorreguieta, A, Semino, C, Jelpi, L
Format: Article
Language:English
Subjects:
Bacteriology
beta-glucans
Biological and medical sciences
biosynthesis
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Rhizobium leguminosarum
Rhizobium leguminosarum bv. viciae
Rhizobium phaseoli
rhizobium viciae
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Summary:Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic beta-(1.2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobility on SDS-polyacrylamide gels (apparent mol. mass of 320 kDa) than the beta-(1,2)-glucan intermediate protein of Rhizobium meliloti. In vivo pulse-label experiments with growing cells of R. legumi-nosarum biovar trifolii RS800 using radioactive glucose showed that few species of cyclic beta-(1,2)-glucans were synthesized and up to 30% were substituted with charged non-glycosidic residues, probably sn-1-phosphoglycerol.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00272135